Journal: The Journal of Biological Chemistry

Article Title: Leucine 7 is a key residue for mutant huntingtin–induced mitochondrial pathology and neurotoxicity in Huntington's disease

doi: 10.1016/j.jbc.2025.108297

Figure Lengend Snippet: HTT-Q73-L7A mutation mitigates HTT-Q73-induced mitochondrial dysfunction and neurodegeneration in vivo . A , schematic diagram of the injection of AAV2/5 in mouse striatum. B and C , Western blotting and quantitative analysis of the expression of flag-tagged HTT-Q23, HTT-Q73, and HTT-Q73-L7A in mouse striatum mitochondrial fractions and WCL at 6 weeks post AAV infection. n = 3 mice. D – I , Western blotting and quantitative analysis of the expression of DARPP32, TFAM, and RNaseH1 in mouse striatum at 6 weeks post AAV infection. n = 6 to 7 mice. One-way ANOVA was conducted in the above results, followed by Tukey's multiple comparison test. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. AAV, adeno-associated virus; HTT-Q73, HTT exon 1 with 73 glutamine repeats; RNaseH1, ribonuclease H1; TFAM, mitochondrial transcription factor A; WCL, whole cell lysates.

Article Snippet: Primary antibodies were diluted 1:1000 and applied as follows: c-Myc (SC-40, Santa Cruz), actin (TA-09, ZSGB-BIO), DRP1 (611,113, BD Biosciences), MFN1 (13798-1-AP, Proteintech), MFN2 (12186-1-AP, Proteintech), ENO3 (15421-1-AP, Proteintech), Matrin3 (12202-2-AP, Proteintech), TFAM (ab131607, Abcam), OPA1 (27733-1-AP, Proteintech), mtCO2 (55070-1-AP, Proteintech), PGC1-α (NBP104676, Novus), cleaved PARP (9541S, CST), ATPB (17247-1-AP, Proteintech), RNaseH1 (15606-1-AP, Proteintech), Poly-γ (A8451, Abclonal), VDAC (55259-1-AP, Proteintech), Cytochrome-C (10993-1-AP, Proteintech), and MnSOD (611580, BD Biosciences).

Techniques: Mutagenesis, In Vivo, Injection, Western Blot, Expressing, Infection, Comparison, Virus

Journal: Journal of Animal Science and Biotechnology

Article Title: Increased maternal consumption of methionine as its hydroxyl analog improves placental angiogenesis and antioxidative capacity in sows

doi: 10.1186/s40104-025-01159-z

Figure Lengend Snippet: Effect of increased consumption of methionine as OHMet on placental proteins. A – C The cluster heat map and volcano map of differential proteins between CON and 1.5S-OHMet, 1.5S-OHMet and 3.0S-OHMet, and 1.5S-OHMet and 3.0S-Met. D Distribution of differential placental proteins in individual samples based on Pearson distance and average clustering. E – H Validation of four differentially expressed proteins from the results of mass spectrometry (MS) and western blotting (WB). TXN, thioredoxin; GSTT1, Glutathione transferase T1; HADHA, enoyl-CoA hydratase; NDUFA3, complex I-B9. The mean abundance of each protein is corrected to β-actin was used as a loading reference ( n = 3). * P < 0.05, ** P < 0.01. Abbreviations: CON = basal diet; 1.5S-OHMet = basal diet + 1.5 g/kg OHMet; 3.0S-OHMet = basal diet + 3.0 g/kg OHMet; 3.0S-Met = basal diet + 3.0 g/kg Met

Article Snippet: Primary antibodies were thioredoxin (TXN, Zen BioScience, 1:1,000), HADHA (Zen BioScience, 1:1,000), NDUFA3 (Zen BioScience, 1:1,000), ENO3 (Zen BioScience, 1:1,000), GSTT1 (Proteintech, 1:1,000), GAPDH (Absin, 1:1,000), β-actin (Cell Signaling Technology, 1:1,000).

Techniques: Biomarker Discovery, Mass Spectrometry, Western Blot

Journal: Journal of Animal Science and Biotechnology

Article Title: Increased maternal consumption of methionine as its hydroxyl analog improves placental angiogenesis and antioxidative capacity in sows

doi: 10.1186/s40104-025-01159-z

Figure Lengend Snippet: Identification of differential expressed proteins in placenta

Article Snippet: Primary antibodies were thioredoxin (TXN, Zen BioScience, 1:1,000), HADHA (Zen BioScience, 1:1,000), NDUFA3 (Zen BioScience, 1:1,000), ENO3 (Zen BioScience, 1:1,000), GSTT1 (Proteintech, 1:1,000), GAPDH (Absin, 1:1,000), β-actin (Cell Signaling Technology, 1:1,000).

Techniques: Coagulation, Permeability, Histone Deacetylase Assay, Transduction